Endocrine Abstracts

Rat and mice bones in vivo and their derived cell cultures in vitro respond sex-specifically to gonadal steroids (GS) by stimulating DNA synthesis (DNA) and the specific activity of creatine kinase (CK; a hormone receptor response marker). Similar results were demonstrated in cultured human male or female-derived bone cells (Obs); female Obs responded only to estradiol-17β (E2) whereas male Obs responded only to dihydrotestosterone (DHT) by stimul...

Ovariectomy of immature female rats, results in significant decreased parameters in different organs. Previously we found that estradiol-17b (E2) restored these parameters in the different organs of ovariectomized female rats (Ovx) to values obtained in intact immature female rats. E2 also stimulated creatine kinase specific activity (CK) a hormonal-responsive marker in organs containing estrogen receptors. In the present study, we compared the effects of...

We have previously reported, that cultured female- derived human Osteoblats (hObs) responded to DT56a (Femarelle). Since the skeletal protective effects of estrogens are not discernible in diabetic women, we sought to test the effects of DT56a on hObs grown in high glucose concentration in the growth medium (HG) as compared to estradiol-17β (E2). We used the stimulation of creatine kinase specific activity (CK), and 3[H] thymidine incorporation into ...